|  | Common 
									Questions and Answers about the BioJect
 1. 
									Why do I need to add more microbes 
									into the field if there are microbes 
									already out there? 2. Do the organisms 
									survive in the irrigation lines?
 3. Do the bacteria 
									survive in the soil / turf environment?
 4. What is the difference 
									between the BioJect and FreshPack? 
									Isn't FreshPack the same as the 
									"bugs in a jug" approach 
									that Eco Soil is opposed to?
 5. How can I be sure 
									that the organisms applied are not 
									harmful to humans and other non-target 
									species?
 6. Is there a risk 
									in over-application of microbes on 
									my golf course?
 7. Does a pesticide 
									application reduce the activity of 
									the microorganism populations being 
									added through the BioJect?
 8. What sorts of 
									university studies are being done 
									with the BioJect?
 9. How do I know 
									the BioJect is actually growing the 
									bacteria and if those bacteria are 
									going out to the field?
 10. What are the 
									space and equipment requirements 
									for the BioJect?
 11. How can the BioJect 
									fit into my budget?
 
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 Answers
 
 1. Why do I need to add more microbes into the field 
							if there are microbes already out there?
 
 Answer: Even though substantial populations 
							exist in the environment, the existence of 
							those organisms that are beneficial 
							may be low in numbers. Modified rooting media, 
							such as USGA specification greens, are primarily 
							sand with low organic matter content, which 
							is a tough environment for the beneficial organisms. 
							Adding substantial numbers of organisms to 
							the soil environment on a frequent basis allows 
							beneficial bacteria to work in the turf environment 
							to help provide better growing conditions.
 
 
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 2. Do the organisms survive in the irrigation lines?
 
 Answer: 
							Based on repeated evaluations of the products 
							that are distributed by the sprinkler heads, 
							there is evidence that not only do the organisms 
							survive, but actually continue to grow in the 
							irrigation lines (though at a much diminished 
							rate). In one study, the bacteria were found 
							to be present without a substantial drop in 
							number at 12 days after injection. This is 
							a difficult evaluation to carry out, yet the 
							initial information gathered demonstrates the 
							existence and relatively even distribution 
							of organisms throughout the irrigation system.
 
 
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  3. Do the bacteria survive in the soil / turf environment?
 
 Answer: Once applied to the turf, 
								microclimate conditions such as moisture, 
								temperature, soil pH, and solar incidence 
								all have a dramatic effect on the survival 
								of an organism. The most difficult challenge 
								facing those introduced into a new environment 
								is competing with the indigenous populations 
								of organisms. Those organisms that already 
								exist within an environment have adapted 
								over many years, possibly millions, and 
								the chance that a new intruder can move 
								in and establish itself is slim.
 The ability to compete for nutrients and for space (by producing 
								antibiotics or parasitizing the intruders) 
								provides the indigenous organism a strong 
								foothold. This is why the BioJect and 
								FreshPack hold such great potential. 
								Through repeated applications the bacteria 
								can get in and get their job done before 
								the local residents have anything to say 
								about it. 
 
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  4. What is 
								the difference between the BioJect and 
								FreshPack? Isn't FreshPack the same 
								as the "bugs in a jug" approach 
								that Eco Soil is opposed to?
 Answer: One 
								Word: Fresh.  Basically, the 
								BioJect provides fresh microbes 
								to the entire course. FreshPack provides 
								fresh microbes to 1-3 acres, usually 
								greens and tees or targeted problem areas. 
								The BioJect delivers more frequent applications 
								(several times per week) at a lower concentration 
								than FreshPack with no labor input. Fresh 
								Pack is sprayed less frequently, usually 
								biweekly, at higher concentrations. 
 Its true that many biological products 
								face short shelf lives, sensitivity to 
								temperature, etc. BioJect overcomes this 
								by producing new organisms just prior 
								to application. FreshPack overcomes this 
								by delivering fresh packaged product 
								that has been carefully maintained and 
								is applied within a few days of receipt.
 
 
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 5. How can I be sure that the organisms 
							applied are not harmful to humans and other 
							non-target species?
 
 Answer: 
							The organisms that are used in the Eco Siol 
							programs do not grow and develop well at body 
							temperature. If they can't grow at body temperature, 
							then the chance that they can invade a human 
							and cause disease symptoms is virtually nonexistent. 
							Concerning other non-targets, the functions 
							that the program bacteria provide are very 
							specific, and the chances that they can cause 
							phytotoxicity to non-target plants or can kill 
							beneficial insects such as ladybugs are extremely 
							small.
 
 
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 6. Is there a risk in over-application of microbes 
							on my golf course?
 
 Answer: 
							Microbial population size is determined by 
							the conditions that are presented by the environment 
							to which they are introduced. When too many 
							bacteria are present based on the supplies 
							of food and other essentials, the excess numbers 
							will simply perish. The environment provides 
							a self-regulating system that helps keep much 
							of the biological portion in check. Another 
							important point is that it would take an incomprehensibly 
							huge number (many times the capacity of the 
							BioJect or FreshPack) to reach a point that 
							could even conceivably overwhelm a soil system.
 
 
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 7. Does a pesticide application reduce the activity 
							of the microorganism populations being added 
							through the BioJect or FreshPack?
 
 Answer: 
							The activity of pesticides is typically specific 
							to the pest that is targeted. Let's take a 
							fungicide for instance; the specificity is 
							very much limited to the control of fungal 
							organisms. In fact, most of the fungicides 
							are targeted for specific types of fungal organisms. 
							For example, Banner performs well in the control 
							of Dollar spot (Sclerotinia homeocarpa) 
							but does not control Pythium (Pythium 
							sp.). Studies performed by Dr. Joe Vargas 
							(Michigan State University) and Dr. Eric Nelson 
							(Cornell University) demonstrated that fungicides 
							had no measurable detrimental effects on bacteria. 
							Recent university studies are showing that 
							the use of fungicides in conjunction with Spotless 
							(a component of the BioJect's BioEnhancement 
							Program) actually extends the period of control 
							beyond which the fungicide alone is capable 
							of providing.
 
 
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 8. What sorts of university studies are being done 
							with the BioJect and FreshPack?
 
 Answer: 
							Over the past few years there has been a rapidly 
							expanding interest in the BioJect among University 
							researchers. Previous research has included 
							Dr. Joe Vargas' work at Michigan State University 
							examining the sensitivity of certain turf diseases 
							to Pseudomonas aureofaciens (spot-less), 
							and Dr. Pete Dernoeden at the University of 
							Maryland evaluating the interaction between 
							beneficial bacteria and chemical fungicides. 
							Researchers evaluating the Eco Soil's microbes 
							include: Dr. Joe Vargas, Michigan State University; 
							Dr. Eric Nelson, Cornell University; Dr. Peter 
							Dernoeden, University of Maryland; John Breshnahan, 
							University of Massachusetts; and Dr. Bruce 
							Clarke, Rutgers University.
 
 
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 9. How do I know the BioJect is actually growing the 
							bacteria and if those bacteria are going out 
							to the field?
 
 Answer: The software on the control 
							system provides a summary of the daily performance 
							of the system, which can show how many days 
							each month the machine performed its fermentation 
							and injection. To assess population levels, 
							we have established testing kits and procedures 
							for samples to be analyzed and certified at 
							independent labs. During the summer of 1997, 
							Eco Soil examined the irrigation water and 
							turf samples of several representative golf 
							courses around the country for the presence 
							of BioJect target bacteria. In nearly every 
							case, we detected substantial levels of the 
							target bacteria in the samples of the irrigation 
							water and turf, showing a relatively even distribution 
							of bacteria throughout the irrigation system. 
							University researchers have also conducted 
							replicated trials on certain courses and have 
							found similar results.
 
 
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 10. What are the space and equipment requirements for 
							the BioJect?
 
 Answer: 
							Any site where the BioJect is to be installed 
							must have: A weatherproof shelter with available 
							space of 90" W x 105" L x 95"H; 
							A dedicated 120 VAC circuit rated at 20 amp 
							service; A drainage output; A source of potable 
							water to fill the BioJect with 25 gallons daily 
							which falls within the following parameters: 
							Pressure 35-65 psi, Flow rate 5-10 gpm, Turbidity 
							<0.5 NTU TDS < 1000 mg/L Ph 6.5 8.5 Iron 
							< 0.3 ppm In most situations, effluent irrigation 
							water is not a problem, as long as the incoming 
							water feeding into the BioJect is of potable 
							quality. This is because the water in which 
							the bacteria grow must meet a more specific 
							set of requirements to maximize the conditions 
							conducive to growth. Once the fermentation 
							cycle is complete and the bacteria are injected 
							into the lines, they can tolerate a wider range 
							of conditions, such as effluent irrigation 
							water.
 
 
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 11. How can the BioJect fit into my budget?
 
 Answer: 
							The BioJect system is designed to provide a 
							biological component to your overall turf management 
							program. The benefits your course receives 
							from the use of the system can either reduce 
							the inputs you currently purchase for your 
							course and/or provide improved turf quality 
							without a dramatic cost increase. After all, 
							the ultimate evaluation of success is the quality 
							of the playing surface produced vs. the dollars 
							spent getting there. The BioJect promotes a 
							better quality turf through:
 
 
								Maintaining 
									disease in a less severe state
Increasing 
									time available to treat turf disease
Improving 
									conditions in roughs and green surrounds
Slowing 
									progression of resistance development 
									to chemicals
 The actual financial 
								savings depend on the area of the country, 
								the disease pressure that the course is 
								under and its fungicide budget. Some customers 
								with as little as a $35,000 annual fungicide 
								budget have completely paid for the system 
								from chemical cost savings by usage reduction 
								in the first year, while other customers 
								with much higher budgets have not. The 
								degree of cost savings depends on how 
								thoroughly the program is integrated into 
								the existing chemical spraying programs, 
								the degree to which the superintendent 
								is willing to push the spray programs, 
								and the disease level that the superintendent 
								is willing to tolerate. 
 
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