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Common Questions and Answers about the BioJect

1. Why do I need to add more microbes into the field if there are microbes already out there?
2. Do the organisms survive in the irrigation lines?
3. Do the bacteria survive in the soil / turf environment?
4. What is the difference between the BioJect and FreshPack? Isn't FreshPack the same as the "bugs in a jug" approach that Eco Soil is opposed to?
5. How can I be sure that the organisms applied are not harmful to humans and other non-target species?
6. Is there a risk in over-application of microbes on my golf course?
7. Does a pesticide application reduce the activity of the microorganism populations being added through the BioJect?
8. What sorts of university studies are being done with the BioJect?
9. How do I know the BioJect is actually growing the bacteria and if those bacteria are going out to the field?
10. What are the space and equipment requirements for the BioJect?
11. How can the BioJect fit into my budget?

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Answers

1. Why do I need to add more microbes into the field if there are microbes already out there?

Answer: Even though substantial populations exist in the environment, the existence of those organisms that are beneficial may be low in numbers. Modified rooting media, such as USGA specification greens, are primarily sand with low organic matter content, which is a tough environment for the beneficial organisms. Adding substantial numbers of organisms to the soil environment on a frequent basis allows beneficial bacteria to work in the turf environment to help provide better growing conditions.


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2. Do the organisms survive in the irrigation lines?

Answer: Based on repeated evaluations of the products that are distributed by the sprinkler heads, there is evidence that not only do the organisms survive, but actually continue to grow in the irrigation lines (though at a much diminished rate). In one study, the bacteria were found to be present without a substantial drop in number at 12 days after injection. This is a difficult evaluation to carry out, yet the initial information gathered demonstrates the existence and relatively even distribution of organisms throughout the irrigation system.


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3. Do the bacteria survive in the soil / turf environment?

Answer: Once applied to the turf, microclimate conditions such as moisture, temperature, soil pH, and solar incidence all have a dramatic effect on the survival of an organism. The most difficult challenge facing those introduced into a new environment is competing with the indigenous populations of organisms. Those organisms that already exist within an environment have adapted over many years, possibly millions, and the chance that a new intruder can move in and establish itself is slim.

The ability to compete for nutrients and for space (by producing antibiotics or parasitizing the intruders) provides the indigenous organism a strong foothold. This is why the BioJect and FreshPack hold such great potential. Through repeated applications the bacteria can get in and get their job done before the local residents have anything to say about it.


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4. What is the difference between the BioJect and FreshPack? Isn't FreshPack the same as the "bugs in a jug" approach that Eco Soil is opposed to?

Answer: One Word: Fresh.

Basically, the BioJect provides fresh microbes to the entire course. FreshPack provides fresh microbes to 1-3 acres, usually greens and tees or targeted problem areas. The BioJect delivers more frequent applications (several times per week) at a lower concentration than FreshPack with no labor input. Fresh Pack is sprayed less frequently, usually biweekly, at higher concentrations.

Its true that many biological products face short shelf lives, sensitivity to temperature, etc. BioJect overcomes this by producing new organisms just prior to application. FreshPack overcomes this by delivering fresh packaged product that has been carefully maintained and is applied within a few days of receipt.


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5. How can I be sure that the organisms applied are not harmful to humans and other non-target species?

Answer: The organisms that are used in the Eco Siol programs do not grow and develop well at body temperature. If they can't grow at body temperature, then the chance that they can invade a human and cause disease symptoms is virtually nonexistent. Concerning other non-targets, the functions that the program bacteria provide are very specific, and the chances that they can cause phytotoxicity to non-target plants or can kill beneficial insects such as ladybugs are extremely small.


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6. Is there a risk in over-application of microbes on my golf course?

Answer: Microbial population size is determined by the conditions that are presented by the environment to which they are introduced. When too many bacteria are present based on the supplies of food and other essentials, the excess numbers will simply perish. The environment provides a self-regulating system that helps keep much of the biological portion in check. Another important point is that it would take an incomprehensibly huge number (many times the capacity of the BioJect or FreshPack) to reach a point that could even conceivably overwhelm a soil system.


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7. Does a pesticide application reduce the activity of the microorganism populations being added through the BioJect or FreshPack?

Answer: The activity of pesticides is typically specific to the pest that is targeted. Let's take a fungicide for instance; the specificity is very much limited to the control of fungal organisms. In fact, most of the fungicides are targeted for specific types of fungal organisms. For example, Banner performs well in the control of Dollar spot (Sclerotinia homeocarpa) but does not control Pythium (Pythium sp.). Studies performed by Dr. Joe Vargas (Michigan State University) and Dr. Eric Nelson (Cornell University) demonstrated that fungicides had no measurable detrimental effects on bacteria. Recent university studies are showing that the use of fungicides in conjunction with Spotless (a component of the BioJect's BioEnhancement Program) actually extends the period of control beyond which the fungicide alone is capable of providing.


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8. What sorts of university studies are being done with the BioJect and FreshPack?

Answer: Over the past few years there has been a rapidly expanding interest in the BioJect among University researchers. Previous research has included Dr. Joe Vargas' work at Michigan State University examining the sensitivity of certain turf diseases to Pseudomonas aureofaciens (spot-less), and Dr. Pete Dernoeden at the University of Maryland evaluating the interaction between beneficial bacteria and chemical fungicides. Researchers evaluating the Eco Soil's microbes include: Dr. Joe Vargas, Michigan State University; Dr. Eric Nelson, Cornell University; Dr. Peter Dernoeden, University of Maryland; John Breshnahan, University of Massachusetts; and Dr. Bruce Clarke, Rutgers University.


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9. How do I know the BioJect is actually growing the bacteria and if those bacteria are going out to the field?

Answer: The software on the control system provides a summary of the daily performance of the system, which can show how many days each month the machine performed its fermentation and injection. To assess population levels, we have established testing kits and procedures for samples to be analyzed and certified at independent labs. During the summer of 1997, Eco Soil examined the irrigation water and turf samples of several representative golf courses around the country for the presence of BioJect target bacteria. In nearly every case, we detected substantial levels of the target bacteria in the samples of the irrigation water and turf, showing a relatively even distribution of bacteria throughout the irrigation system. University researchers have also conducted replicated trials on certain courses and have found similar results.


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10. What are the space and equipment requirements for the BioJect?

Answer: Any site where the BioJect is to be installed must have: A weatherproof shelter with available space of 90" W x 105" L x 95"H; A dedicated 120 VAC circuit rated at 20 amp service; A drainage output; A source of potable water to fill the BioJect with 25 gallons daily which falls within the following parameters: Pressure 35-65 psi, Flow rate 5-10 gpm, Turbidity <0.5 NTU TDS < 1000 mg/L Ph 6.5 8.5 Iron < 0.3 ppm In most situations, effluent irrigation water is not a problem, as long as the incoming water feeding into the BioJect is of potable quality. This is because the water in which the bacteria grow must meet a more specific set of requirements to maximize the conditions conducive to growth. Once the fermentation cycle is complete and the bacteria are injected into the lines, they can tolerate a wider range of conditions, such as effluent irrigation water.


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11. How can the BioJect fit into my budget?

Answer: The BioJect system is designed to provide a biological component to your overall turf management program. The benefits your course receives from the use of the system can either reduce the inputs you currently purchase for your course and/or provide improved turf quality without a dramatic cost increase. After all, the ultimate evaluation of success is the quality of the playing surface produced vs. the dollars spent getting there. The BioJect promotes a better quality turf through:
  • Maintaining disease in a less severe state
  • Increasing time available to treat turf disease
  • Improving conditions in roughs and green surrounds
  • Slowing progression of resistance development to chemicals

The actual financial savings depend on the area of the country, the disease pressure that the course is under and its fungicide budget. Some customers with as little as a $35,000 annual fungicide budget have completely paid for the system from chemical cost savings by usage reduction in the first year, while other customers with much higher budgets have not. The degree of cost savings depends on how thoroughly the program is integrated into the existing chemical spraying programs, the degree to which the superintendent is willing to push the spray programs, and the disease level that the superintendent is willing to tolerate.


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